top of page

Featured Publications

All Publications

* Equal contribution, # Corresponding author
Mechanisms and functions of large extracellular vesicle biogenesis
Stephanie F. Rutter, Jascinta P. Santavanond, Alan Y. Hsu, Taylon F. Silva, Dolores Di Vizio, Ivan K. H. Poon
2026
Nature Cell Biology
Read Paper
RABEP1 regulates neutrophil migration via endosomal recycling and actin polymerization
Daniel H Kim, Ramizah Syahirah, Conwy Zheng, Chang Ding, Alan Y Hsu, Tyler Pikes, Zhaolun Liang, Sheng Liu, Linlin Li, Xiaoping Bao, David Umulis, Jun Wan, Qing Deng
2026
Journal of Leukocyte Biology
Read Paper
Neutrophil death-more than meets the eye
Alan Y. Hsu*, Qingxiang Huang*, Fei Liu, Arumugam Balasubramanian, Hongbo R. Luo
2025
Experimental Hematology
Read Paper
Protocol to isolate and quantify large aging neutrophil-derived vesicles
Alan Y. Hsu*, Qingxiang Huang*, Sizhou Feng, Hongbo R. Luo
2025
Star Protocols
Read Paper
Neutrophil-derived vesicles control complement activation to facilitate inflammation resolution
Alan Y. Hsu*, Qingxiang Huang*, Xiong Pi, Jianing Fu, Krishnan Raghunathan, Laxman Ghimire, Arumugam Balasubramanian, Xuemei Xie, Hongbo Yu, Fabien Loison, Viraga Haridas, Jiali Zha, Fei Liu, Shin-young Park, Kamal Bagale, Qian Ren, Yuping Fan, Yi Zheng, Jose A. Cancelas, Li Chai, Sean R. Stowell, Kanchao Chen, Rong Xu, Xiaoxue Wang, Yuanfu Xu, Lianghui Zhang, Tao Cheng, Fengxia Ma, Jay R. Thiagarajah, Hao Wu, Sizhou Feng, and Hongbo R. Luo
2025
Cell
Read Paper
IQGAP1 Influences Neutrophil Maturation and Its Effector Functions
Lifei Hou, Alan Hsu, Hongbo Luo, Koichi Yuki
2025
European Journal of Immunology
Read Paper
Gasdermin E dictates inflammatory responses by controlling the mode of neutrophil death
Fengxia Ma, Laxman Ghimire, Qian Ren, Yuping Fan, Tong Chen, Arumugam Balasubramanian, Alan Hsu, Fei Liu, Hongbo Yu, Xuemei Xie, Rong Xu & Hongbo R. Luo
2024
Nature Communications
Read Paper
The effect of transient, constricted migration on neutrophil intracellular bacteria-killing capability
Peng Wu*, Alan Y. Hsu*, Ting Peng, Wenxue Zhao, Fei Liu, Zhaoran Zhang, Laxman Ghimire, Arumugam Balasubramanian, Xuemei Xie, Jiajia Li, Shin-Young Park, Fengxia Ma, Cheng Li, and Hongbo R. Luo
2024
Immunity
Read Paper
The palmitoylation of gasdermin D directs its membrane translocation and pore formation during pyroptosis
Arumugam Balasubramanian, Alan Y. Hsu, Laxman Ghimire, Muhammad Tahir, Pascal Devant, Pietro Fontana, Gang Du, Xing Liu, Dang Fabin, Hiroto Kambara, Xuemei Xie, Fei Liu, Tomoya Hasegawa, Rong Xu, Hongbo Yu, Mei Chen, Steven Kolakowski, Sunia Trauger, Martin Røssel Larsen, Wenyi Wei, Hao Wu, Jonathan C. Kagan, Judy Lieberman, Hongbo R. Luo
2024
Science immunology
Read Paper
Neutrophil-specific depletion of gasdermin D does not protect against murine sepsis
Fei Liu, Laxman Ghimire, Arumugam Balasubramanian, Alan Hsu, Zhaoran Zhang, Hongbo Yu, Fenxia Ma, Hongbo R Luo
2023
Blood
Read Paper
Neutrophil Lifespan Extension with CLON-G and an In Vitro Spontaneous Death Assay
Yuping Fan , Yan Teng , Fu Tong Liu , Fengxia Ma, Alan Y Hsu, Sizhou Feng, Hongbo R Luo
2023
Journal of Visualized Experiments (JoVE)
Read Paper
Method for Visualization of Emergency Granulopoiesis in the Zebrafish Embryo
Ramizah Syahirah, Jennifer Beckman, Hanna Malik, Alan Y. Hsu, and Qing Deng
2023
Zebrafish
Read Paper
NLRP3 is essential for neutrophil polarization and chemotaxis in response to leukotriene B4 gradient
Stijn Van Bruggen, Pierre-André Jarrot, Eline Thomas, Casey E. Sheehy, Camila M. S. Silva, Alan Y. Hsu, Pierre Cunin, Peter A. Nigrovic, Edgar R. Gomes, Hongbo R. Luo, Clare M. Waterman, and Denisa D. Wagner
2023
Proceedings of the National Academy of Sciences
Read Paper
Rora Regulates Neutrophil Migration and Activation in Zebrafish
Alan Y. Hsu*, Tianqi Wang*, Ramizah Syahirah*, Sheng Liu, Kailing Li, Weiwei Zhang, Jiao Wang, Ziming Cao, Simon Tian, Sandro Matosevic, Chris Staiger, Jun Wan, and Qing Deng
2022
Frontiers in Immunology
Read Paper
Chemically-defined generation of human hemogenic endothelium and definitive hematopoietic progenitor cells
Yun Chang, Ramizah Syahirah, Stephanie N. Oprescu, Xuepeng Wang, Juhyung Jung, Scott H. Cooper, Sandra Torregrosa-Allen, Bennett D. Elzey, Alan Y. Hsu, Lauren N. Randolph, Yufei Sun, Shihuan Kuang, Hal E. Broxmeyer, Qing Deng, Xiaojun Lian, Xiaoping Bao
2022
Biomaterials
Read Paper
Enzyme-Responsive Peptide Thioesters for Targeting Golgi Apparatus
Weiyi Tan, Qiuxin Zhang, Monica C Quiñones-Frías, Alan Y. Hsu, Yichi Zhang, Avital Rodal, Pengyu Hong, Hongbo R. Luo, Bing Xu
2022
Journal of the American Chemical Society
Read Paper
A curious case of cyclin-dependent kinases in neutrophils
Ramizah Syahirah*, Alan Y. Hsu*, Qing Deng
2022
Journal of Leukocyte Biology
Read Paper
Heterogeneity of neutrophils in cancer: one size does not fit all
Song Chen, Qingyu Zhang, Lisha Lu, Chunhui Xu, Jiajia Li, Jiali Zha, Fengxia Ma, Hongbo R. Luo#, Alan Y. Hsu#
2022
Cancer Biology & Medicine
Read Paper
Isolation of Human Neutrophils from Whole Blood and Buffy Coats
Alan Y. Hsu, Zhicheng Peng, Hongbo Luo, Fabien Loison
2021
Journal of Visualized Experiments (JoVE)
Read Paper
A robust and flexible CRISPR/Cas9-based system for neutrophil-specific gene inactivation in zebrafish
Yueyang Wang*, Alan Y. Hsu*, Eric M. Walton, Sung Jun Park, Ramizah Syahirah, Tianqi Wang, Wenqing Zhou, Chang Ding, Abby Pei Lemke, GuangJun Zhang, David M. Tobin and Qing Deng
2021
Journal of Cell Science
Read Paper
Mitofusin-2 regulates leukocyte adhesion and β2 integrin activation
Wei Liu*, Alan Hsu*, Yueyang Wang, Tao Lin, Hao Sun, Joel Pachter, Alex Groisman, Matthew Imperioli, Fernanda Yungher, Liang Hu, Penghua Wang, Qing Deng, and Zhichao Fan
2021
Journal of Leukocyte Biology
Read Paper
Discovery of Small Molecules That Target the Phosphatidylinositol (3,4,5) Trisphosphate (PIP3)-Dependent Rac Exchanger 1 (P-Rex1) PIP3-Binding Site and Inhibit P-Rex1–Dependent Functions in Neutrophils
Jennifer N. Cash, Naincy R. Chandan, Alan Y. Hsu, Prateek V. Sharma, Qing Deng, Alan V. Smrcka, and John J.G. Tesmer
2020
Molecular Pharmacology
Read Paper
Mitofusin 2 regulates neutrophil adhesive migration and the actin cytoskeleton
Wenqing Zhou*, Alan Y. Hsu*, Yueyang Wang*, Ramizah Syahirah, Tianqi Wang, Jacob Jefferies, Haroon Mohammad, Xu Wang, Mohamed N. Seleem, David Umulis, Qing Deng
2020
Journal of Cell Science
Read Paper
miRNA-223 at the crossroads of inflammation and cancer
Jacob Jeffries, Wenqing Zhou*, Alan Y. Hsu*, and Qing Deng
2019
Cancer Letters
Read Paper
Identification and characterization of permissive cells to dengue virus infection in human hematopoietic stem and progenitor cells
Alan Y. Hsu, Tzu‐Chuan Ho, Mei‐Ling Lai, Sia Seng Tan, Tsai‐Yun Chen, Meed Lee, Yu‐Wen Chien, Ya‐Ping Chen, Guey Chuen Perng
2019
Transfusion
Read Paper
Inducible overexpression of zebrafish microRNA-722 suppresses chemotaxis of human neutrophil like cells
Alan Y. Hsu, Sheng Liu, Kent A. Brasseale, Ramizah Sabri, Jun Wan, Qing Deng
2019
Molecular immunology
Read Paper
Phenotypical microRNA screen reveals a noncanonical role of CDK2 in regulating neutrophil migration
Alan Y. Hsu, Decheng Wang, Sheng Liu, Justice Lu, Ramizah Syahirah, David A. Bennin, Anna Huttenlocher, Jun Wan, Qing Deng
2019
Proceedings of the National Academy of Sciences
Read Paper
MicroRNA-223 Suppresses the Canonical NF-κB Pathway in Basal Keratinocytes to Dampen Neutrophilic Inflammation
Wenqing Zhou, Arpita S. Pal, Alan Y. Hsu, Theodore Gurol, Xiaoguang Zhu, Sarah E. Wirbisky, Jennifer L. Freeman, Andrea L. Kasinski, Qing Deng
2018
Cell Reports
Read Paper
Development and Characterization of an Endotoxemia Model in Zebra Fish
Alan Y. Hsu, Theodore Gurol, Tiago J. Sobreira, Sheng Zhang, Natalie Moore, Chufan Cai, Zhong-Yin Zhang and Qing Deng
2018
Frontiers in Immunology
Read Paper
Overexpression of microRNA-722 fine-tunes neutrophilic inflammation by inhibiting Rac2 in zebrafish
Alan Y. Hsu*, Decheng Wang*, Theodore Gurol*, Wenqing Zhou, Xiaoguang Zhu, Hsiu-Yi Lu, Qing Deng
2017
Disease Models & Mechanisms
Read Paper
Infectious dengue vesicles derived from CD61+ cells in acute patient plasma exhibited a diaphanous appearance
Alan Yi-Hui Hsu, Shang-Rung Wu, Jih-Jin Tsai, Po-Lin Chen, Ya-Ping Chen, Tsai-Yun Chen, Yu-Chih Lo, Tzu-Chuan Ho, Meed Lee, Min-Ting Chen, Yen-Chi Chiu & Guey Chuen Perng
2015
Scientific Reports
Read Paper
Pathogenic Parameters Derived from Activated Platelets in Dengue Patients
Jih-Jin Tsai, Po-Chih Chen, Li-Teh Liu, Ko Chang, Ju-Han Yao, Hui-Mien Hsiao, Kristina B Clark, Yu Han Chen, Alan Yi-Hui Hsu, Guey-Chuen Perng
2013
Tropical Medicine & Surgery
Read Paper
bottom of page